ALTERNATE-CURRENT MACHINERY.

Canonical and alternate functions of the microRNA biogenesis machinery.

The canonical microRNA (miRNA) biogenesis pathway requires two RNaseIII enzymes: Drosha and Dicer. To understand their functions in mammals in vivo, we engineered mice with germline or tissue-specific inactivation of the genes encoding these two proteins. Changes in proteomic and transcriptional profiles that were shared in Dicer- and Drosha-deficient mice confirmed the requirement for both enz...

Alternate Test of RF Mixers by Current Signatures

This paper describes an alternate test methodology for radio-frequency mixers. The methodology is based on current signatures obtained by sampling the filtered supply current when the circuit-under-test is stimulated by a single tone sinusoidal. Sampling of supply current instead of voltage eliminates the parasitics and loading due to test circuitry on the signal path, hence provides a non-inva...

Functional characterization of alternate optimal solutions of Escherichia coli's transcriptional and translational machinery.

The constraint-based reconstruction and analysis approach has recently been extended to describe Escherichia coli's transcriptional and translational machinery. Here, we introduce the concept of reaction coupling to represent the dependency between protein synthesis and utilization. These coupling constraints lead to a significant contraction of the feasible set of steady-state fluxes. The subs...

The Molecular Machinery of Resurgent Sodium Current Revealed

Some TTX-sensitive sodium channels open transiently during recovery from inactivation, generating a "resurgent" sodium current that flows immediately following action potentials. In this issue of Neuron, Grieco and colleagues present evidence that resurgent sodium current results from a novel form of inactivation in which the cytoplasmic tail of the beta4 subunit acts as a classic open-channel ...

استرابیسم متناوب روزانه ( Alternate day squint )